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Three plasmids (pGL1124, pGL1224 and pGL1217) had been created to allow the substitution of 1 allele of CYC9, is unfold with the tsetse fly and will cause trypanosomiasis in individuals and animals. Both the life cycle and mobile cycle with the parasite are elaborate. Trypanosomes have eleven cdc2-related kinases (CRKs) and 10 cyclins, an unusually